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WebKligman maximization test in guinea-pigs. Following repeated dietary dosing, the liver was the main target organ in mice, rats and dogs. Hepatotoxicity was manifest as increased liver weight, liver enlargement and centrilobular hepatocellular hypertrophy, as well as alterations in clinical chemistry (elevated serum levels of liver WebGuinea Pig Maximization Test (GPMT) The procedure described by Magnusson & Kligman (1) was followed, comprising intradermal induction with FCA on day 0; topical induction on day 7; a subsequent challenge on day 21; and rechallenge on day 35 by closed-patch tests to the flanks of the animal (Fig. 1). WebCurrent European Community (Annex V) guidelines recommend the use of 20 test animals in the guinea pig maximisation test for skin sensitisation. The suitability, for classification and labelling purposes, of reducing the number of test animals has been examined by analysing the results of 40 studies submitted to the Health and Safety Executive, and by the use of a … cool surname for girls